Background: RNF111 (Ring finger protein 111) acts in the NODAL pathway of mesoderm patterning during embryonic development. It is an E3 ubiquitin-protein ligase which promotes the ubiquitination of inhibitory SMADs such as SMAD7, induces their proteasomal degradation and thereby enhances the transcriptional activity of TGF-beta and BMP. It also induces degradation of the transriptional repressors SnoN and c-Ski. RNF111, therefore, effectively enhances TGF-beta signaling through simultaneous down-regulation of two distinct types of negative regulators, Smad7 and SnoN/c-Ski.
Description: Rabbit polyclonal to RNF111
Immunogen: KLH conjugated synthetic peptide derived from RNF111
Specificity: ·Reacts with Human, Mouse and Rat.
·Isotype: IgG
Application: ·Western blotting: 1/100-500. Predicted Mol wt: 109 kDa;
·Immunohistochemistry (Paraffin/frozen tissue section): 1/50-200;
·Immunocytochemistry/Immunofluorescence: 1/100;
·Immunoprecipitation: 1/50;
·ELISA: 1/500;
·Optimal working dilutions must be determined by the end user.
