Background: GPAT2 (glycerol-3-phosphate acyltransferase 2, mitochondrial), also known as Gm116 or xGPAT1, is an 801 amino acid mitochondrial multi-pass membrane protein belonging to the GPAT/DAPAT family. GPAT2 is highly expressed in testis with lower levels in heart, liver, kidney, spleen and adipose cells. Inhibited by N-ethylmaleimide (NEM), GPAT2 esterifies an acyl-group from acyl-ACP to the sn-1 position of glycerol-3-phosphate, an essential step in glycerolipid biosynthesis. GPAT2 contain a HXXXXD motif, which is critical for acyltransferase activity and may constitute the binding site for the phosphate moiety of the glycerol-3-phosphate. Three isoforms of GPAT2 exist due to alternative splicing events.
Description: Rabbit polyclonal to GPAT2
Immunogen: KLH conjugated synthetic peptide derived from GPAT2
Specificity: ·Reacts with Human, Mouse, Pig, Dog and Rat.
·Isotype: IgG
Application: ·Western blotting: 1/100-500. Predicted Mol wt: 89 kDa;
·Immunohistochemistry (Paraffin/frozen tissue section): 1/50-200;
·Immunocytochemistry/Immunofluorescence: 1/100;
·Immunoprecipitation: 1/50;
·ELISA: 1/500;
·Optimal working dilutions must be determined by the end user.
