Background: Intraflagellar transport is mediated by a variety of intraflagellar transport proteins (IFTs) that work in tandem to mediate ciliary and flagellar process assembly. Endogenous IFT proteins are most highly expressed within the inner segment, around the basal body, and within the outer segment.Additionally, IFT proteins are localized in discrete particles along the entire length of the axoneme. IFT proteins are divided into two subcomplexes, A and B, which contain at least 6 or 11 subunits, respectively. IFT-A proteins are associated with retrograde transport, whereas IFT-B proteins are thought to be involved in structure because, in their absence, cilia and flagella may be truncated, or completely absent. IFT172 (intraflagellar transport 172), also known as SLB, wim or osm-1, is a 1,749 amino acid protein that belongs to the IFT172 family and localizes to the cilium. Containing fourteen TPR repeats and nine WD repeats, IFT172 is required for the maintenance and formation of cilia. IFT172 plays an indirect role in Shh signaling, with cilia being required for all activity of the hedgehog pathway.
Description: Rabbit polyclonal to IFT172
Immunogen: KLH conjugated synthetic peptide derived from IFT172
Specificity: ·Reacts with Human, Mouse, Pig, Dog and Rat.
·Isotype: IgG
Application: ·Western blotting: 1/100-500. Predicted Mol wt: 197 kDa;
·Immunohistochemistry (Paraffin/frozen tissue section): 1/50-200;
·Immunocytochemistry/Immunofluorescence: 1/100;
·Immunoprecipitation: 1/50;
·ELISA: 1/500;
·Optimal working dilutions must be determined by the end user.
