Background: The Rho family of GTP-binding proteins plays a role in the development of neuronal structure. The activation of the GTP-bound form is regulated by GTPase-activating proteins, which stimulate GTP hydrolysis, leading to inactivation. GMIP (Gem-interacting protein) is a 970 amino acid protein that stimulates the GTPase activity of RhoA in vitro and in vivo. GMIP interacts with Gem through its N-terminus and has a Rho GTPase-activating protein domain at its C-terminus. GMIP is able to inhibit RhoA function, leading to Actin cytoskeletal reorganization in vivo. Encoded by a gene that maps to human chromosome 19p13.11, GMIP contains one phorbol-ester/DAG-type zinc finger and one Rho-GAP domain.
Description: Rabbit polyclonal to GMIP
Immunogen: KLH conjugated synthetic peptide derived from GMIP
Specificity: ·Reacts with Human, Mouse, Pig, Dog and Rat.
·Isotype: IgG
Application: ·Western blotting: 1/100-500. Predicted Mol wt: 107 kDa;
·Immunohistochemistry (Paraffin/frozen tissue section): 1/50-200;
·Immunocytochemistry/Immunofluorescence: 1/100;
·Immunoprecipitation: 1/50;
·ELISA: 1/500;
·Optimal working dilutions must be determined by the end user.
