Background: The claudin superfamily consists of structurally related proteins that are important structural and functional components of tight junctions. Three classes of proteins are known to localize to tight junctions, including the claudins, Occludin and Junction adhesion molecules (JAMs). Claudins, which consist of four transmembrane domains and two extracellular loops make up tight junction strands. Emerging evidence suggests that the Claudin family of proteins regulates transport through tight junctions via differential discrimination for solute size and charge. Claudin-25, also known as CLDN25, is a 229 amino acid multi-pass membrane protein that localizes to tight junctions and belongs to the claudin family. Claudin-25 is involved in obliteration of intracellular space at the tight junction via calcium-independent cell-adhesion and is encoded by a gene that maps to human chromosome 11q23.2.
Description: Rabbit polyclonal to Claudin 25
Immunogen: KLH conjugated synthetic peptide derived from Claudin 25
Specificity: ·Reacts with Human, Mouse and Rat.
·Isotype: IgG
Application: ·Western blotting: 1/100-500. Predicted Mol wt: 25 kDa;
·Immunohistochemistry (Paraffin/frozen tissue section): 1/50-200;
·Immunocytochemistry/Immunofluorescence: 1/100;
·Immunoprecipitation: 1/50;
·ELISA: 1/500;
·Optimal working dilutions must be determined by the end user.
