Background: Cytochrome c oxidase subunit VIc (COX6c), also designated oxidative phosphorylation (OxPhos) complex IV, subunit VIc, is one of the structural subunits of the mitochondrial respiratory chain encoded by nuclear genes. Cytochrome c oxidase is a hetero-oligomeric enzyme composed of 13 subunits localized to the mitochondrial inner membrane and is the terminal enzyme complex of the electron transport chain. Complex IV catalyzes the reduction of molecular oxygen to water. The energy released is used to transport protons across the mitochondrial inner membrane. The resulting electrochemical gradient is necessary for the synthesis of ATP. Complex IV contains 13 polypeptides; COX1, COX2 and COX3 (MTCO1-3) make up the catalytic core and are encoded by mtDNA while subunits IV, Va, Vb, VIa, VIb, VIc, VIIa, VIIb, VIIc and VIII are nuclear-encoded. The nuclear-encoded subunits function in the regulation and assembly of the complex. The human COX6c protein shares 77% sequence identity with mouse COX6c. Studies indicate that the COX6c gene is upregulated in prostate cancer cells. The human COX6c gene maps to chromosome 8q22.2; a pseudogene, COX6CP1 has been found on chromosome 16p12.
Description: Rabbit polyclonal to COX6c
Immunogen: KLH conjugated synthetic peptide derived from COX6c
Specificity: ·Reacts with Human, Mouse, Pig, Dog and Rat.
·Isotype: IgG
Application: ·Western blotting: 1/100-500. Predicted Mol wt: 9 kDa;
·Immunohistochemistry (Paraffin/frozen tissue section): 1/50-200;
·Immunocytochemistry/Immunofluorescence: 1/100;
·Immunoprecipitation: 1/50;
·ELISA: 1/500;
·Optimal working dilutions must be determined by the end user.
