Background: Members of the Complexin protein family promote SNARE (soluble N-ethylmaleimide-sensitive factor attachment protein receptors) precomplex formation by binding to Syntaxin via an α-helical domain. Complexins are important regulators of transmitter release at a late step in calcium-dependent neurotransmitter release or immediately after the calcium-triggering step of fast synchronous transmitter release. Neurons lacking Complexins show reduced transmitter release efficiency due to decreased calcium sensitivity of the synaptic secretion process. Complexin-3, also known as CPXIII, CPX-III, Nbla11589 or CPLX3, is a 158 amino acid member of the complexin/synaphin family. Complexin-3 is involved in the regulation of synaptic vesicle exocytosis. Complexin-3 binds to the SNARE core complex containing SNAP 25, VAMP-2 and Syntaxin 1.
Description: Rabbit polyclonal to CPLX3
Immunogen: KLH conjugated synthetic peptide derived from CPLX3
Specificity: ·Reacts with Human, Mouse and Rat.
·Isotype: IgG
Application: ·Western blotting: 1/100-500. Predicted Mol wt: 18 kDa;
·Immunohistochemistry (Paraffin/frozen tissue section): 1/50-200;
·Immunocytochemistry/Immunofluorescence: 1/100;
·Immunoprecipitation: 1/50;
·ELISA: 1/500;
·Optimal working dilutions must be determined by the end user.
