Background: Synaptic nuclear envelope protein-1 (Syne-1) is a member of the Golgi- and nuclear envelope-localized spectrin family, and it facilitates retrograde vesicular trafficking from the Golgi to the ER. Syne-1 is a 8,797 amino acid protein that contains several spectrin repeats similar to those in dystrophin and utrophin, two tandem calponin homology domains at its N-terminus, as well as a domain that is homologous to the C-terminal of Klarsicht, a Drosophila protein associated with nuclei and necessary for a few nuclear migrations. Syne-1 localizes to the Golgi apparatus and nuclear envelope until cytokinesis, when it migrates to the central spindle and midbody, where it functions together with KIF3B to assist the accumulation of the membrane vesicles at the spindle midbody.
Description: Rabbit polyclonal to Nesprin 1
Immunogen: KLH conjugated synthetic peptide derived from Nesprin 1
Specificity: ·Reacts with Human, Mouse and Rat.
·Isotype: IgG
Application: ·Western blotting: 1/100-500. Predicted Mol wt: 112 kDa;
·Immunohistochemistry (Paraffin/frozen tissue section): 1/50-200;
·Immunocytochemistry/Immunofluorescence: 1/100;
·Immunoprecipitation: 1/50;
·ELISA: 1/500;
·Optimal working dilutions must be determined by the end user.
