Background: The replication of lagging strand DNA requires several enzymatic steps that eventually lead to the generation of Okazaki fragments; small DNA strands that are attached by DNA Ligase. DNA2 is a helicase/nuclease that is essential for genome stability and DNA metabolism. DNA2 acts as a DNA-dependent ATPase that unwinds duplex DNA, thereby producing single-standed DNA which serves as a template for DNA replication. The human homolog of DNA2, which is known as both DNA2 and DNA2L (DNA2-like helicase), is a 1,060 amino acid protein that functions as a helicase that is involved in DNA replication, DNA repair and chromatin dynamics. Specifically, DNA2L is thought to play a role in the maturation and elongation of Okazaki fragments, thereby facilitating replication of the lagging strand. Mutations in the gene encoding DNA2L are lethal, suggesting that proper DNA2L function is crucial for cell viability. Three isoforms of DNA2L exist due to alternative splicing events.
Description: Rabbit polyclonal to DNA2
Immunogen: KLH conjugated synthetic peptide derived from DNA2
Specificity: ·Reacts with Human, Mouse and Rat.
·Isotype: IgG
Application: ·Western blotting: 1/100-500. Predicted Mol wt: 120 kDa;
·Immunohistochemistry (Paraffin/frozen tissue section): 1/50-200;
·Immunocytochemistry/Immunofluorescence: 1/100;
·Immunoprecipitation: 1/50;
·ELISA: 1/500;
·Optimal working dilutions must be determined by the end user.
