Background: DRAM (damage-regulated autophagy modulator) is a multi-pass membrane protein that belongs to the TMEM77 family of proteins and localizes to the lysosome membrane. DRAM is a highly conserved protein across many species and contains six transmembrane domains and an endoplasmic reticulum (ER) signal peptide. Its expression is induced by both p53 and p73, and it acts as a key player that is required (but not sufficient) for p53-induced autophagy and apoptosis. Although its expression is also induced by p73, DRAM is dispensable for p73-mediated apoptosis. As is suggested by its lysosomal localization, DRAM may participate in the degradation of proteins or in trafficking through the secretory pathway. In addition, DRAM expression is downregulated in human cancers, implying a profound role for DRAM in tumor development.
Description: Rabbit polyclonal to DRAM
Immunogen: KLH conjugated synthetic peptide derived from DRAM
Specificity: ·Reacts with Human, Mouse and Rat.
·Isotype: IgG
Application: ·Western blotting: 1/100-500. Predicted Mol wt: 26 kDa;
·Immunohistochemistry (Paraffin/frozen tissue section): 1/50-200;
·Immunocytochemistry/Immunofluorescence: 1/100;
·Immunoprecipitation: 1/50;
·ELISA: 1/500;
·Optimal working dilutions must be determined by the end user.
