Background: TRAM2 (translocation associated membrane protein 2) is a 370 amino acid multi-pass membrane protein containing one TLC (TRAM/LAG1/CLN8) domain. TRAM2 interacts with SERCA2B and COL1A1 and is necessary for collagen type I synthesis. TRAM2 facilitates proper folding of collagen by coupling the activity of SERCA2B, an ER Ca2+ pump, and the activity of translocons, thereby increasing local Ca2+ concentration at the site of collagen synthesis and stimulating molecular chaperones that are involved in collagen folding. It is suggested that TRAM2 is regulated by RUNX2 in a BMP-dependent manner and may play a role in the overall osteogenic function of RUNX2. RUNX2 is essential for skeletal mineralization in which it stimulates osteoblast differentiation of mesenchymal stem cells, promotes chondrocyte hypertrophy and contributes to endothelial cell migration and vascular invasion of developing bones.
Description: Rabbit polyclonal to TRAM2
Immunogen: KLH conjugated synthetic peptide derived from TRAM2
Specificity: ·Reacts with Human, Mouse and Rat.
·Isotype: IgG
Application: ·Western blotting: 1/100-500. Predicted Mol wt: 43 kDa;
·Immunohistochemistry (Paraffin/frozen tissue section): 1/50-200;
·Immunocytochemistry/Immunofluorescence: 1/100;
·Immunoprecipitation: 1/50;
·ELISA: 1/500;
·Optimal working dilutions must be determined by the end user.
