Background: Nidogens are highly conserved proteins present in vertebrate and invertebrate basement membranes. Nidogens connect the Laminin and Collagen IV networks and integrate other proteins into the membrane. In mammals, two Nidogen proteins, Nidogen and Nidogen-2, interact at comparable levels with Collagen I, IV and Perlecan, serving to stabilize basement membranes and playing a major role in embryogenesis. The two isoforms have a similar shape, consisting of three globular domains, and co-localize in vessel walls and other basement membrane zones. Nidogen-2 is a cell adhesion protein glycosylated at nitrogen and oxygen sites, and is widely distributed in basement membranes in heart, placenta, bone and, to a lesser extent, in pancreas, kidney and skeletal muscle. Plays a role in the maintenance of the undifferentiated state of germ cells regulating the spermatogonia cell cycle and inducing a prolonged transit in G1 phase. Affects cell proliferation probably by repressing translation of specific mRNAs. Maintains the germ cell lineage by suppressing both Bax-dependent and -independent apoptotic pathways. Essential in the early stage embryo to protect the migrating primordial germ cells (PGCs) from apoptosis.
Description: Rabbit polyclonal to Nanos3
Immunogen: KLH conjugated synthetic peptide derived from Nanos3
Specificity: ·Reacts with Human, Mouse and Rat.
.·Isotype: IgG
Application: ·Western blotting: 1/100-500. Predicted Mol wt: 19 kDa;
·Immunohistochemistry (Paraffin/frozen tissue section): 1/50-200;
·Immunocytochemistry/Immunofluorescence: 1/100;
·Immunoprecipitation: 1/50;
·ELISA: 1/500;
·Optimal working dilutions must be determined by the end user.
