Background: The type II cAMP-protein kinase (PKA) is a multifunctional kinase with a broad range of substrates. Specificity of PKA signaling is thought to be mediated by the compartmentalization of the kinase to specific sites within the cell. To maintain this specific localization, the regulatory (R) subunits (RI and RII) of PKA interact with specific R-anchoring proteins designated AKAPs (A-kinase anchoring proteins). AKAP 7 ( A-kinase anchor protein 7), also known as AKAP18, is a 104 amino acid protein that belongs to the AKAP family. AKAP 7 is expressed in brain, heart, lung, pancreas and skeletal muscle. AKAP 7 binds PKA to the plasma membrane, and permits functional coupling to the L-type calcium channel. Four isoforms exist due to alternative splicing events. It has been suggested that the © isoform binds RI and may be responsible for positioning PKA via RI and/or RII to regulate PKA-mediated gene transcription in both somatic cells and oocytes.
Description: Rabbit polyclonal to AKAP7
Immunogen: KLH conjugated synthetic peptide derived from AKAP7
Specificity: ·Reacts with Human, Mouse and Rat.
·Isotype: IgG
Application: ·Western blotting: 1/100-500. Predicted Mol wt: 37 kDa;
·Immunohistochemistry (Paraffin/frozen tissue section): 1/50-200;
·Immunocytochemistry/Immunofluorescence: 1/100;
·Immunoprecipitation: 1/50;
·ELISA: 1/500;
·Optimal working dilutions must be determined by the end user.
