Background: G protein-coupled receptors (GPCRs), which are characterized by containing seven transmembrane å helices, elicit G protein-mediated signaling cascades in response to a variety of stimuli. The opsin subfamily, which represents approximately 90 percent of all GPCRs, is comprised of photoreceptors that are activated by light. It includes the red, green and blue-sensitive opsins and rhodopsin. The opsin subfamily consists of an apoprotein covalently linked to 11-cis-retinal, which undergoes isomerization upon the absorption of photons. This isomerization leads to a conformational change of the protein, which results in the activation of hundreds of G proteins. Color is perceived in humans by three pigments, which localize to retinal cone photoreceptor cells. They are the blue-, green- and red-sensitive opsins, which are encoded by OPN1SW, OPN1MW and OPN1LW, respectively. Mutations in the OPN1MW and OPN1LW encoded opsins lead to the X-linked disorders protanopia and deuteranopia, respectively. Mutations in the OPN1SW encoded opsin leads to tritanopia, an autosomal dominant disorder, which is characterized by decreased sensitivity to blue light.
Description: Rabbit polyclonal to Opn1mw
Immunogen: KLH conjugated synthetic peptide derived from Opn1mw
Specificity: ·Reacts with Human, Mouse and Rat.
.·Isotype: IgG
Application: ·Western blotting: 1/100-500. Predicted Mol wt: 40 kDa;
·Immunohistochemistry (Paraffin/frozen tissue section): 1/50-200;
·Immunocytochemistry/Immunofluorescence: 1/100;
·Immunoprecipitation: 1/50;
·ELISA: 1/500;
·Optimal working dilutions must be determined by the end user.
