Background: GATAD2B (GATA zinc finger domain containing 2B), also known as P66beta, is a 593 amino acid protein that contains one GATA-type zinc finger and localizes to discrete foci within the nucleus. Expressed ubiquitously, GATAD2B is thought to function as a transcriptional repressor that interacts with the methyl-CpG-binding protein MBD3, a component of the MeCP1 complex. MeCP1 is a multi-subunit complex that represses transcription via histone deacetylation and nucleosomal remodeling, thereby condensing chromatin structure and preventing transcription. GATAD2B interacts with and recruits MBD3 to specific areas within the nucleus, thereby participating in MeCP1 complex-mediated transcriptional repression. GATAD2B is expressed in various cancer cell lines, suggesting a possible role in carcinogenesis.
Description: Rabbit polyclonal to p66 beta
Immunogen: KLH conjugated synthetic peptide derived from p66 beta
Specificity: ·Reacts with Human, Mouse and Rat.
·Isotype: IgG
Application: ·Western blotting: 1/100-500. Predicted Mol wt: 66 kDa;
·Immunohistochemistry (Paraffin/frozen tissue section): 1/50-200;
·Immunocytochemistry/Immunofluorescence: 1/100;
·Immunoprecipitation: 1/50;
·ELISA: 1/500;
·Optimal working dilutions must be determined by the end user.
