Background: Pulmonary surfactant is a complex mixture of phospholipids and proteins secreted from airway epithelial cells (type II cells) in alveoli which reduces the surface tension at the alveolar air liquid interface, providing alveolar stability necessary for normal ventilation. Four distinct proteins isolated from pulmonary surfactant are termed surfactant proteins A, B, C and D. SP A (28 - 36 kDa) and SP D (43 kDa) are collagenous carbohydrate binding proteins, whereas SP B (8 - 9 kDa) and SP C (4 kDa) are non collagenous hydrophobic proteins. SP C is a fragment of a much larger precursor protein of 21 kD. The precursor contains an exceedingly hydrophobic region of 34 amino acids that comprises most of the mature SP C, and a unique poly-valine domain.
Description: Rabbit polyclonal to SP-C
Immunogen: KLH conjugated synthetic peptide derived from SP-C
Specificity: ·Reacts with Human, Mouse, Rabbit and Rat.
·Isotype: IgG
Application: ·Western blotting: 1/100-500. Predicted Mol wt: 21 kDa;
·Immunohistochemistry (Frozen/paraffin tissue section): 1/100-200;
·Immunocytochemistry: 1/100;
·ELISA: 1/500;
·Optimal working dilutions must be determined by the end user.
