Background: bNOS (nitric oxide synthase from brain) produces nitric oxide which is a messenger molecule with diverse functions throughout the body. In the brain and peripheral nervous system, nitric oxide displays many properties of a neurotransmitter. bNOS is an inhibitory transmitter for non-adrenergic and non cholinergic nerves in the colorectum. NOS is classified under three types: Type I NOS is found in neurons and is constitutively expressed and Ca2+/calmodulin dependent. It is called NOS-1, neuronal NOS (nNOS), brain NOS (bNOS), cerebral / constitutive / Ca2+ regulated NOS (cNOS). bNOS is present in skeletal muscles and its absence in Duchenne’s muscular dystrophy accounts for its symptoms of the disease. Type II NOS is 130kDa protein known as macrophage NOS (mNOS) or inducible NOS (iNOS). It is Ca2+/calmodulin independent and is expressed in activated macrophages and stimulated glial cells. Type III NOS is a 135kDa protein called endothelial NOS (eNOS).
Description: Rabbit polyclonal to NOS1
Immunogen: KLH conjugated synthetic peptide derived from NOS1
Specificity: ·Reacts with Human, Mouse, Rabbit and Rat.
·Isotype: IgG
Application: ·Western blotting: 1/500-1000. Predicted Mol wt: 160 kDa;
·Immunohistochemistry (Frozen/paraffin tissue section): 1/100-500;
·Immunocytochemistry: 1/200-500;
·ELISA: 1/500;
· Optimal working dilutions must be determined by the end user.
