Background: This protein promotes cell death. Successfully competes for the binding to Bcl-X(L), Bcl-2 and Bcl-W, thereby affecting the level of heterodimerization of these proteins with BAX. It can reverse the death repressor activity of Bcl-X(L), but not that of Bcl-2 (By similarity). It appears to act as a link between growth factor receptor signaling and the apoptotic pathways. Phosphorylated on one or more of Ser-75, Ser-91, Ser-99, Ser-118 and Ser-134 in response to survival stimuli, which blocks its pro-apoptotic activity. Phosphorylation on Ser-99 or Ser-75 promotes heterodimerization with 14-3-3 proteins. This interaction then facilitates the phosphorylation at Ser-118, a site within the BH3 motif, leading to the release of Bcl-X(L) and the promotion of cell survival. Ser-99 is the major site of AKT/PKB phosphorylation, Ser-118 the major site of protein kinase A (CAPK) phosphorylation. Ser-75 is phosphorylated by AKT/PKB, protein kinase A and PIM2.
Description: Rabbit polyclonal to phospho-Bad(Ser118).
Immunogen: A synthesized phosphopeptide derived from human Bad around the phosphorylation site of Ser 118. All Sites: Human: Ser118; Mouse: Ser155; Rat: Ser156.
Specificity: ·Reacts with Human, Mouse and Rat.
·Isotype: IgG
Application: ·Western blotting: 1/100-500. Predicted Mol wt: 18 kDa;
·Immunohistochemistry (Frozen/paraffin tissue section): 1/100-200;
·Immunocytochemistry: 1/100;
·ELISA: 1/500;
·Optimal working dilutions must be determined by the end user.
