Background: DENND1B (DENN/MADD domain containing 1B), also known as C1orf218 or FAM31B, is a 426 amino acid protein that contains a dDENN domain, DENN domain and uDENN domain and exists as five isoforms as a result of alternative splicing. The DENND1B protein contains phosphorylation sites on Tyr-475, Tyr-484, Ser-514 and Ser-652 and is thought to activate Rab35 in an endocytic pathway trafficking Shiga toxin to the trans-Golgi network. The gene encoding DENND1B maps to human chromosome 1, the largest human chromosome which spans about 260 million base pairs and makes up 8% of the human genome, where it may be involved in asthma susceptibility. Other notable genes located on chromosome 1 include LMNA, which is associated with the rare aging disease Hutchinson-Gilford progeria, and the MUTYH gene, which is partially responsible for familial adenomatous polyposis. Stickler syndrome, Parkinsons, Gaucher disease and Usher syndrome.
Description: Rabbit polyclonal to DENND1B
Immunogen: KLH conjugated synthetic peptide derived from DENND1B
Specificity: ·Reacts with Human, Mouse and Rat.
·Isotype: IgG
Application: ·Western blotting: 1/100-500. Predicted Mol wt: 48 kDa;
·Immunohistochemistry (Frozen/paraffin tissue section): 1/100-500;
·Immunocytochemistry: 1/100-200;
·ELISA: 1/500;
·Optimal working dilutions must be determined by the end user.
