Background: Dynactin, a multisubunit complex, is a cytoplasmic Dynein-interacting protein that functions as the ”receptor” for the Dynein microtubule motor. Dynactin/ Dynein binding may be required for most, if not all, cytoplasmic Dynein-driven activities and is thought to contribute to the functional diversity of Dynein. Enriched in neurons, Dynactin also binds to microtubules and has been shown to function in diverse processes, including organelle transport, formation of the mitotic spindle and cytokinesis. Dynactin 3, also known as Dynactin complex subunit 22 kDa subunit or p22, is a 186 amino acid subunit of the Dynactin complex. Dynactin 3 localizes to punctate cytoplasmic structures, to the centrosome during interphase and to the kinetochores and spindle poles throughout mitosis. Ubiquitously expressed, Dynactin 3 is found at highest levels in pancreas and muscle.
Description: Rabbit polyclonal to DCTN3
Immunogen: KLH conjugated synthetic peptide derived from DCTN3
Specificity: ·Reacts with Human, Mouse, Dog and Rat.
·Isotype: IgG
Application: ·Western blotting: 1/100-500. Predicted Mol wt: 21 kDa;
·Immunohistochemistry (Paraffin/frozen tissue section): 1/100-500;
·Immunocytochemistry/Immunofluorescence: 1/100;
·Immunoprecipitation: 1/50;
·ELISA: 1/500;
·Optimal working dilutions must be determined by the end user.
