Background: The H-H-H-H-H-H motif is used as a tag on many recombinant proteins to facilitate purification. The antibody recognizes the His-tag fused to the amino- or carboxy- termini of targeted proteins in transfected or transformed cells.
Description: Rabbit polyclonal to His tag
Immunogen: KLH conjugated synthetic peptide 6×His tag
Specificity: ·Reacts with fusion protein, HHHHHH tagged.
·Isotype: IgG
Application: ·Western blotting: 1/100-500;
·Immunohistochemistry (Paraffin/frozen tissue section): 1/50-200;
·Immunocytochemistry/Immunofluorescence: 1/100;
·Immunoprecipitation: 1/50;
·ELISA: 1/500;
·Optimal working dilutions must be determined by the end user.
