Background: Phosducin-like protein (PhLP, PDCL) is an ethanol-responsive modulator of heterotrimeric G proteins. The protein shares extensive amino acid sequence homology with phosducin (Phd), a phosphoprotein expressed in retina and pineal gland. Both PhLP and Phd regulate G-protein signaling by binding to the beta-gamma subunits of G proteins. PhLP interacts with G beta-gamma via a short C-terminal binding site. Additionally, PhLP acts as a substrate for GRK2 phosphorylation at the same C-terminal binding site between residues 195 and 218. PhLPs may participate directly in the regulation of calcium-evoked exocytosis in adrenal medullary chromaffin cells. Glycosylated PhLP regulates opioid receptor function in mouse brain.
Description: Rabbit polyclonal to PHLP
Immunogen: KLH conjugated synthetic peptide derived from PHLP
Specificity: ·Reacts with Human, Mouse and Rat.
·Isotype: IgG
Application: ·Western blotting: 1/100-500. Predicted Mol wt: 34 kDa;
·Immunohistochemistry (Paraffin/frozen tissue section): 1/100-500;
·Immunocytochemistry/Immunofluorescence: 1/100;
·Immunoprecipitation: 1/50;
·ELISA: 1/500;
·Optimal working dilutions must be determined by the end user.
