Background: The phosphorylation and dephosphorylation of proteins on serine and threonine residues is an essential means of regulating a broad range of cellular functions in eukaryotes, including cell division, homeostasis and apoptosis. A group of proteins that are intimately involved in this process are the serine/threonine (Ser/Thr) protein kinases. PRP4 kinase, also known as PRPF4B, PRP4H or PRP4K, is a 1,007 amino acid nuclear protein that contains one protein kinase domain and belongs to the Ser/Thr protein kinase family. Expressed ubiquitously, PRP4 kinase functions to catalyze the ATP-dependent phosphorylation of target proteins and is thought to play a role in pre-mRNA splicing, possibly participating in the spliceosome C complex. Human PRP4 kinase shares 98% sequence identity with its mouse counterpart, suggesting a conserved role between species.
Description: Rabbit polyclonal to PRPF4B
Immunogen: KLH conjugated synthetic peptide derived from PRPF4B
Specificity: ·Reacts with Human, Mouse and Rat.
·Isotype: IgG
Application: ·Western blotting: 1/100-500. Predicted Mol wt: 117 kDa;
·Immunohistochemistry (Paraffin/frozen tissue section): 1/50-200;
·Immunocytochemistry: 1/100;
·Immunoprecipitation: 1/50;
·ELISA: 1/500;
·Optimal working dilutions must be determined by the end user.
