Background: IL-27 triggers expansion of antigen-specific naive CD4+ T cells and promotes polarization towards a Th1 phenotype with expression of γ-interferon. IL-27 contributes to the development of an adaptive immune response through its action on CD4+ T cells and also directly acts on cells of the innate immune system. IL-27 protein levels increase upon activation of antigen-presenting cells. IL-27 protein induces orphan cytokine receptor WSX-1/TCCR-dependent clonal expansion of naive but not memory CD4+ T cells. IL-27 signaling through TCCR/WSX-1 induces phosphorylation of Stat1-5. The predicted 243 amino acid human IL-27 protein, which is 73% identical to the mouse protein, contains an N-terminal signal peptide, several O-glycosylation sites and a stretch of 13 glutamate residues between helices C and D.
Description: Rabbit polyclonal to IL-27
Immunogen: KLH conjugated synthetic peptide derived from IL-27
Specificity: ·Reacts with Human, Mouse and Rat.
·Isotype: IgG
Application: ·Western blotting: 1/100-500. Predicted Mol wt: 27 kDa;
·Immunohistochemistry (Paraffin/frozen tissue section): 1/50-200;
·Immunocytochemistry: 1/100;
·Immunoprecipitation: 1/50;
·ELISA: 1/500;
·Optimal working dilutions must be determined by the end user.
