Background: The Bcl-2 gene was isolated at the chromosomal breakpoint of t(14;18)-bearing follicular B cell lymphomas.Bcl-2 blocks cell death following a variety of stimuli and confers a death-sparing effect to certain hematopoietic cell lines following growth factor withdrawal.Bcl-2 appears to function in several subcellular locations yet lacks any known motifs that would confer insight into its mechanism of action.A more recently identified protein,designated Bax p21(i.e., Bcl-associated X protein), has extensive amino acid homology with Bcl-2 and both homodimerizes and forms heterodimers with Bcl-2.Overexpression of Bax accelerates apoptotic death induced by cytokine deprivation in an IL-3 dependent cell line and Bax also counters the death repressor activty of Bcl-2.
Description: Mouse monoclonal to Bcl2
Immunogen: KLH conjugated synthetic peptide derived from Bcl2
Specificity: ·Recognizes Human Bcl2 in Immunohistochemiscal staining and Western blot.
·Isotype: IgG
Application: ·Western blotting: 1/100-500. Predicted Mol wt: 26 kDa;
·Immunohistochemistry (Paraffin/frozen tissue section): 1/50-200;
·Immunocytochemistry/Immunofluorescence: 1/100;
·Immunoprecipitation: 1/50;
·ELISA: 1/500;
·Optimal working dilutions must be determined by the end user.
