Background: Ubiquitination is an important mechanism through which three classes of enzymes act in concert to target short-lived or abnormal proteins for destruction. The three classes of enzymes involved in ubiquitination are the ubiquitin-activating enzymes (E1s), the ubiquitin-conjugating enzymes (E2s) and the ubiquitin-protein ligases (E3s). HEI10 (human enhancer of invasion 10), also known as CCNB1IP1 (cyclin B1 interacting protein 1), is a 277 amino acid protein that localizes to the nucleus and contains one RING-type zinc finger. Expressed at high levels in heart and present at lower levels in kidney, liver, brain, lung and placenta, HEI10 functions as an E3 ubiquitin-protein ligase that modulates cyclin B levels and plays an important role in proper cell cycle progression. HEI10 is subject to post-translational modification, including ubiquitination and phosphorylation.
Description: Rabbit polyclonal to CCNB1IP1
Immunogen: KLH conjugated synthetic peptide derived CCNB1IP1
Specificity: ·Reacts with Human, Mouse and Rat.
·Isotype: IgG
Application: ·Western blotting: 1/100-500. Predicted Mol wt: 32 kDa;
·Immunohistochemistry (Paraffin/frozen tissue section): 1/50-200;
·Immunocytochemistry/Immunofluorescence: 1/100;
·Immunoprecipitation: 1/50;
·ELISA: 1/500;
·Optimal working dilutions must be determined by the end user.
