Background: LEPREL2 (leprecan-like protein 2), also known as P3H3 (prolyl 3-hydroxylase 3) or protein B, is a 736 amino acid protein belonging to the leprecan family, a family of collagen prolyl hydroxlysases required for proper collagen biosynthesis, folding and assembly. LEPREL2 contains one Fe2OG dioxygenase domain and four TPR repeats. LEPREL2 has prolyl 3-hydroxylase activity catalyzing the post-translational formation of 3-hydroxyproline in -Xaa-Pro-Gly-sequences in collagens, especially types IV and V. Localized in the endoplasmic reticulum, LEPREL2 is weakly expressed in heart, lung, ovary and skeletal muscle. Two isoforms of LEPREL2 are produced by alternative splicing events. The gene encoding LEPREL2 maps to human chromosome 12p13.31 and mouse chromosome 6 F2.
Description: Rabbit polyclonal to LEPREL2
Immunogen: KLH conjugated synthetic peptide derived from LEPREL2
Specificity: ·Reacts with Human, Mouse, Pig, Dog and Rat.
·Isotype: IgG
Application: ·Western blotting: 1/100-500. Predicted Mol wt: 82 kDa;
·Immunohistochemistry (Paraffin/frozen tissue section): 1/50-200;
·Immunocytochemistry/Immunofluorescence: 1/100;
·Immunoprecipitation: 1/50;
·ELISA: 1/500;
·Optimal working dilutions must be determined by the end user.
