Background: The Drosophila melanogaster Flightless I gene is required for normal cellularization of the syncytial blastoderm in early embryogenesis and in the structural organization of indirect flight muscle. The Flightless I protein contains an Actin-binding domain with homology to the gelsolin family and is likely to be involved in Actin cytoskeletal rearrangements. Flightless I also contains an N-terminal leucine-rich repeat protein-protein interaction domain. The Flightless I protein localizes predominantly to the nucleus and translocates to the cytoplasm following serum stimulation. In cells stimulated to migrate, the Flightless I protein co-localizes with b-tubulin- and Actin-based structures. The human FLI gene is mapped within the Smith-Magenis microdeletion region of chromosome 17 at 17p11.2. Smith-Magenis syndrome is characterized by short stature, brachydactyly, developmental delay, dysmorphic features, sleep disturbances and behavioral problems.
Description: Rabbit polyclonal to Flightless 1
Immunogen: KLH conjugated synthetic peptide derived from Flightless 1
Specificity: ·Reacts with Human, Mouse and Rat.
·Isotype: IgG
Application: ·Western blotting: 1/100-500. Predicted Mol wt: 144 kDa;
·Immunohistochemistry (Paraffin/frozen tissue section): 1/50-200;
·Immunocytochemistry/Immunofluorescence: 1/100;
·Immunoprecipitation: 1/50;
·ELISA: 1/500;
·Optimal working dilutions must be determined by the end user.
