Background: In Greek mythology the fate known as Klotho is a goddess who spins the thread of life. In mice, a deficiency in Klotho (kl) gene expression leads to various systemic phenotypes resembling human aging. Characteristics of the Klotho deficient mouse include arteriosclerosis, osteoporosis, ectopic calcification, and skin atrophy together with growth retardation, short life-span and infertility. Mice deficient in Klotho show barely detectable amounts of white adipose tissue yet their brown adipose tissue (BAT) is comparably the same as in a normal genotype, suggesting that Klotho influences adipose differentiation. Mouse and human Klotho gene products are both characteristic type I transmembrane proteins that are approximately 80% homologous. The amino terminal extracellular domain has two internal repeats, known as KL-1 and KL-2, which have partial sequence homology to β-glucosidases and lactase glycosylceramidase, suggesting a role for Klotho in sphingolipid metabolism. The human Klotho gene maps to chromosome 13q12 and encodes a 1012 amino acid protein that is abundant in the kidney and brain. Chronic renal failure (CRF) patients express lower levels of Klotho mRNA and protein in the kidneys.
Description: Rabbit polyclonal to Klotho
Immunogen: KLH conjugated synthetic peptide derived from Klotho
Specificity: ·Reacts with Human, Mouse and Rat.
·Isotype: IgG
Application: ·Western blotting: 1/100-500. Predicted Mol wt: 116 kDa;
·Immunohistochemistry (Frozen/paraffin tissue section): 1/100-500;
·Immunocytochemistry: 1/100-200;
·ELISA: 1/500;
·Optimal working dilutions must be determined by the end user.
