Background: PRPF4, also known as U4/U6 snRNP 60 kDa protein or WD splicing factor Prp4, is a 522 amino acid protein containing seven WD repeats. Localized to the nucleus, PRPF4 colocalizes with spliceosomal snRNPs and is involved in pre-RNA splicing. PRPF4 is part of a heteromeric complex which interacts with U4/U5/U6 tri-snRNP complex and is stable in the absence of RNA. PRPF4 interacts directly with PPIH, PRPF18 and PRPF3. The gene that encodes PRPF4 maps to human chromosome 9, which consists of about 145 million bases and encodes nearly 900 genes. Considered to play a role in gender determination, deletion of the distal portion of 9p can lead to development of male to female sex reversal, the phenotype of a female with a male X,Y genotype. Notably, chromosome 9 encompasses the largest interferon family gene cluster. Chromosome 9 is partnered with chromosome 22 in the translocation leading to the aberrant production of BCR-ABL fusion protein often found in leukemias.
Description: Rabbit polyclonal to PRPF4
Immunogen: KLH conjugated synthetic peptide derived from PRPF4
Specificity: ·Reacts with Human, Mouse and Rat.
·Isotype: IgG
Application: ·Western blotting: 1/100-500. Predicted Mol wt: 58 kDa;
·Immunohistochemistry (Paraffin/frozen tissue section): 1/50-200;
·Immunocytochemistry: 1/100;
·Immunoprecipitation: 1/50;
·ELISA: 1/500;
·Optimal working dilutions must be determined by the end user.
