Background: The Wnt genes belong to a family of protooncogenes with at least 13 known members that are expressed in species ranging from Drosophila to man. The name Wnt denotes the relationship of this family to the Drosophila segment polarity gene “wingless” and to its vertebrate ortholog, Int-1, a mouse protooncogene. Transcription of Wnt family genes appears to be developmentally regulated in a precise temporal and spatial manner. The Wnt genes encode cysteine-rich putative glycoproteins, which have features typical of secreted growth factors. Wnt-8b was named on the basis of the very high sequence similarity (approximately 90% identity) of the inferred protein to those encoded by the Xenopus and zebrafish Wnt-8b genes. The human and mouse ex-pression patterns appear to be identical and are restricted to the developing brain, with the great majority of expression being found in the developing forebrain. Expression in the developing hippocampus may suggest a role for Wnt-8b in patterning of this region. The gene which encodes Wnt-8b maps to human chromosome 10q24.
Description: Rabbit polyclonal to Wnt8A
Immunogen: KLH conjugated synthetic peptide derived from Wnt8A
Specificity: ·Reacts with Human, Mouse and Rat.
·Isotype: IgG
Application: ·Western blotting: 1/100-500. Predicted Mol wt: 39 kDa;
·Immunohistochemistry (Paraffin/frozen tissue section): 1/50-200;
·Immunocytochemistry: 1/100;
·Immunoprecipitation: 1/50;
·ELISA: 1/500;
·Optimal working dilutions must be determined by the end user.
