Background: The fidelity of protein synthesis requires efficient discrimination of amino acid substrates by aminoacyl-tRNA synthetases. Aminoacyl-tRNA synthetases function to catalyze the aminoacylation of tRNAs by their corresponding amino acids, thus linking amino acids with tRNA-contained nucleotide triplets. HARS2(histidyl-tRNA synthetase 2), also known as HO3, HARSL or HARSR, is a 506 amino acid protein that localizes to the mitochondrial matrix and belongs to the class II aminoacyl-tRNA synthetase family. Highly expressed in kidney, heart and skeletal muscle with lower levels present in liver and brain, HARS2 functions in the ATP-dependent synthesis of histidyl-transfer RNA, playing an accessory role in the regulation of protein synthesis. The gene encoding HARS2 maps to human chromosome 5, which contains 181 million base pairs and comprises nearly 6% of the human genome.
Description: Rabbit polyclonal to HARS2
Immunogen: KLH conjugated synthetic peptide derived from HARS2
Specificity: ·Reacts with Human, Mouse and Rat.
·Isotype: IgG
Application: ·Western blotting: 1/100-500. Predicted Mol wt: 57 kDa;
·Immunohistochemistry (Paraffin/frozen tissue section): 1/50-200;
·Immunocytochemistry/Immunofluorescence: 1/100;
·Immunoprecipitation: 1/50;
·ELISA: 1/500;
·Optimal working dilutions must be determined by the end user.
